cleavage and polyadenylation specificity factor การใช้

• At the effector domain, it interacts and inhibits cleavage and polyadenylation specificity factor ( CPSF30 ).
• Likewise, it interacts with cytoplasmic polyadenylation factors, including Cleavage and polyadenylation specificity factor and CPEB, and with target mRNAs.
• Cleavage requires four multisubunit complexes, namely cleavage and polyadenylation specificity factor ( CPSF ), cleavage stimulation factor ( CstF ), cleavage factors Im and IIm ( CFIm and CFIIm ), along with a single subunit poly ( A ) polymerase ( PAP ).
• The protein is the final addition to a large protein complex that also contains smaller assemblies known as the cleavage and polyadenylation specificity factor ( CPSF ) and cleavage stimulatory factor ( CtSF ) and its binding is a necessary prerequisite to the cleavage of the 3'end of the pre-mRNA . After cleavage of the 3'signaling region that directs the assembly of the complex, polyadenylate polymerase ( PAP ) adds the polyadenine tail to the new 3'end.